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KMID : 0880220080460030338
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Journal of Microbiology
2008 Volume.46 No. 3 p.338 ~ p.343
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Two Forms of Vibrio vulnificus Metalloprotease VvpE are Secreted via the Type II General Secretion System
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Park Jong
Ryu So-Yeon
Shin Sung-Hee
Kim Choon-Mee
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Abstract
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Vibrio vulnificus has been known to secrete one form of metalloprotease VvpE (45 kDa) that is cleaved to 34 kDa-VvpE and 11 kDa-C-terminal propeptide via extracellular autoproteolysis. However, we found that extracellular secretion of both the 34 and 45 kDa forms of VvpE began in the early growth phase; moreover, 34 kDa-VvpE existed as the major form in V. vulnificus cell lysates and culture supernatants. In addition, extracellular secretion of both 34 and 45 kDa-VvpE was blocked by mutation of the pilD gene, which encodes for the type IV leader peptidase/N-methyltransferase of the type II general secretion system, and the blocked VvpE secretion was recovered by in trans-complementation of the wild-type pilD gene. These results indicate that 34 kDa-VvpE is the major form secreted along with 45 kDa-VvpE from the early growth phase via the PilD-mediated type II general secretion system.
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KEYWORD
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Vibrio vulnificus, metalloprotease, general secretion system
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